Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of diagnostic ultrasound on corneal apoptosis in rats

Cong Liu, Xin Yu, Yun Kou

Department of Ultrasound Diagnositics, General Hospital of Northern Theater Command, Shenyang, Liaoning, China;

For correspondence:- Yun Kou Email: gc856869@126.com Tel:+8618292831303

Accepted: 29 June 2020 Published: 30 September 2020

Citation: Liu C, Yu X, Kou Y. Effect of diagnostic ultrasound on corneal apoptosis in rats. Trop J Pharm Res 2020; 19(9):1947-1951 doi: 10.4314/tjpr.v19i9.21

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of diagnostic ultrasound on corneal apoptosis in rats.

Methods: 24 male rats were randomly divided into 4 groups: control group, 10, 20 and 30 min group. The eyeballs of rats were irradiated continuously for different time lengths by Siemens ACUSON S2000 color Doppler ultrasound diagnostic instrument. 24 hours later, the animals were killed and the corneas were taken for Tunel apoptosis detection. The apoptosis rates of corneal epithelial cells, stromal cells and endothelial cells were calculated.

Results: Apoptotic cells were detected in corneal epithelial cells, stromal cells and endothelial cells of normal rats. There was no significant difference between the 10 min group and the control group (P>0.05). The apoptosis rate of 20 min and 30 min groups was significantly higher than that of the control group. With the extension of irradiation time, the apoptosis rate of corneal epithelial cells, stromal cells and endothelial cells increased.

Conclusion: 20 min of rat eyeball irradiated by diagnostic ultrasound can increase the apoptosis of corneal cells, and the apoptosis is aggravated with the prolongation of ultrasound irradiation time.

Keywords: Cornea; Ultrasonography; Apoptosis; Epithelial cells